TEM1 Antibody

• Catalog Number: orb1240092
• Category: Antibodies
• Description: TEM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-TEM-1 antibody (orb1240092) was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1. The immunogen is located within amino acids 40-90 of TEM-1
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 81kDObserved: 90 kD (post-modifications: 27 O-linked glycosylations)
• Target: CD248
• UniProt ID: Q9HCU0
• NCBI: NP_065137
• Storage: Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: TEM1 Antibody: TEM1, CD164L1, TEM1, Endosialin, Tu
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation in Human Colon Tissue. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240092, (A, 0.5 µg/ml; B, 1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240088, (2 µg/mL), TEM-1 orb1240092, (1 µg/mL), and GAPDH (0.02 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Tissue. Loading: 15 µg of lysates per lane. Antibodies: TEM-1 orb1240092, (0.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of TEM-1 in Human Colon Tissue. Immunohistochemical analysis of paraffin-embedded Human Colon tissue using anti-TEM-1 antibody (orb1240092) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of TEM-1 in Human Colon Cells. Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with orb1240092 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).