TEM1/CD248 Antibody

• Catalog Number: orb1537860
• Category: Antibodies
• Description: TEM1/CD248 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A 14 AA synthetic peptide near the C-Terminus of human TEM1. The immunogen is located within the last 50 AA of TEM1.
• Concentration: 1 mg/ml
• Dilution range: ELISA, IF, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml)
• Conjugation: Unconjugated
• Target: TEM1 / CD248
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS containing 0.02% Sodium Azide. PBS containing 0.02% Sodium Azide
• Alternative names: CD248, CD248 antigen, CD164 sialomucin-like 1, CD2
• Note: For research use only
• Application notes: Further information: TEM1 antibody can be used for detection of TEM1 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 48, 83 kDa Observed: 50, 88 kDa
• Expiration Date: 12 months from date of receipt.

Immunofluorescence of TEM1 in Human Colon cells with TEM1 antibody at 20 ug/ml.

Western blot of TEM1 in human colon tissue lysate with TEM1 antibody at (A) 0.5 and (B) 1 ug/ml. Below: Immunohistochemistry of TEM1 in human colon tissue with TEM1 antibody at 2.5 ug/ml.