TCF4 Antibody (N-term)

• Catalog Number: orb1930369
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB21346
• Tested applications: FC, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 71308 Da
• Target: This TCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-73 amino acids from the N-terminal region of human TCF4.
• UniProt ID: P15884
• NCBI: NP_001230161.1, NP_001230156.1, NP_001077431.1, NP_001230157.1, NP_001230159.1, NP_001230165.1, NP_001230162.1, NP_003190.1, NP_001230164.1, NP_001230163.1, NP_001230155.1, NP_001230160.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Transcription factor 4, TCF-4, Class B basic helix
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

TCF4 Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

All lanes: Anti-TCF4 Antibody (N-term) at 1:1000 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: K562 whole cell lysate. Lane 3: Mouse cerebellum whole tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 71 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.