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Catalog Number | orb1937490 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB19650 |
Tested applications | WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:2000, WB: 1:2000, WB: 1:1000 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 67919 Da |
Target | This TCF7L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-90 amino acids from the N-terminal region of human TCF7L2. |
UniProt ID | Q9NQB0 |
NCBI | NP_001139746.1, NP_001139756.1, NP_001139755.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Transcription factor 7-like 2, HMG box transcripti Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
All lanes: Anti-TCF7L2 Antibody (N-term) at 1:2000 dilution. Lane 1: HCT116 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: mouse cerebellum lysate. Lane 5: mouse heart lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-TCF7L2 Antibody (N-term) at 1:2000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
TCF7L2 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35 ug/lane). This demonstrates the TCF7L2 antibody detected the TCF7L2 protein (arrow).