Tau Antibody

SKU: orb699467

Description

Rabbit Anti-Human Truncated Tau dGAE (297-391) Polyclonal

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution was sufficient for detection of tau in 15 μg of mouse, rat brain cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Human Tau AA297-391 (dGAE) Fibril (SPR-461)
• Target: Tau
• Molecular Weight: 10.2 kDa
• Purification: Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tau, MAPT, Microtubule-associated protein tau, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, TAU_HUMAN, TAU, MAPTL, MTBT1, MTBT2, RNPTAU, PHF-tau, PHF tau, Paired helical filament tau, Paired helical filament-tau, Neurofibrillary tangle protein, DDPAC, FTDP 17, MSTD, PPND, PPP1R103, Protein phosphatase 1 regulatory subunit 103, G protein beta1/gamma2 subunit interacting factor 1, AI413597, AW045860, FLJ31424, MGC134287, MGC138549, MGC156663, dGAE tau fibril, Truncated Tau Fragment (AA297-391), 95-amino acid tau protein fragment, Truncated Tau Protein

Western blot analysis of Mouse, Rat Brain showing detection of Multiple bands due to different isoforms/aggregation states Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Mouse brain (15 ug). Lane 2: Rat brain (15 ug). Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: Multiple bands due to different isoforms/aggregation states. Other Band (s): 50 kDa, 65 kDa, 100 kDa.

Dot blot analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Recombinant Tau Monomers and Fibrils. Species: Human. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking.

Quick Database Links

Gene Symbol

Tau

UniProt

P10636

NCBI Gene ID

4137

RefSeq (Protein):NP_005901.2