Tau-4 Mouse Monoclonal Antibody

• Catalog Number: orb2563035
• Category: Antibodies
• Description: Tau-4 Mouse Monoclonal Antibody
• Target: MAPT
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2a
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: Recombinant human Tau-F protein (1-441/441aa)
• UniProt ID: P10636
• MW: 46/79 kDa
• Tested applications: IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:200-1000, IHC-F=1:200-1000, IF=1:200-1000
• Antibody Type: Primary Antibody
• Clone Number: 1A8
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAPT; Microtuble-associted protein Tau; AI413597;
• Research Area: Neurodegenerative Disease, Neuroscience, TAU Pathw
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

(Negative control) Paraformaldehyde-fixed, paraffin embedded Human Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4Monoclonal Antibody, Unconjugated (orb2563035) at 1:1000 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

(Negative control) Paraformaldehyde-fixed, paraffin embedded Mouse Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

(Negative control) Paraformaldehyde-fixed, paraffin embedded Rat Spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Breast, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebellum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Left Parietal Lobe, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:600 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Tau-4 Monoclonal Antibody, Unconjugated (orb2563035) at 1:800 overnight at 4°C, followed by conjugation to the SP Kit (Mouse) and DAB staining.