Tau Antibody

SKU: orb612846

Description

Mouse monoclonal antibody to Tau

Research Area

Cell Biology, Disease Biomarkers, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: DOT, ELISA, ICC, IF, WB
• Dilution Range: WB (1:1000)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  A 1:1000 dilution of SMC-607 was sufficient for detection of Tau 2N4R P301S Fibril in 20 ug of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1D5
• Immunogen: Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils
• Target: Tau
• Purification: Protein G Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated
• Concentration: 1mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Tau, TAU, TAU_HUMAN, MAPT, MAPTL, Microtubule-associated protein tau, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Neurofibrillary tangle protein, Paired helical filament tau, Paired helical filament-tau, PHF tau, PHF-tau, DDPAC, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, MSTD, Mtapt, MTBT1, MTBT2, PPND, PPP1R103, Protein phosphatase 1 regulatory subunit 103, RNPTAU, AI413597, AW045860, FLJ31424, MGC134287, MGC138549, MGC156663

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5. Tissue: iPSC-derived neurons. Species: Human. Fixation: 4% PFA. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:100 for Overnight at 4°C. Counterstain: DAPI at 1:5000 for 5 minutes at RT in the dark. Magnification: 40X.

Western Blot analysis of Human, Mouse Breast Cancer Cell line, Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5. Lane 1: MW Marker. Lane 2: Human T-47d (10ug). Lane 3: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 1D5. Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.

Quick Database Links

Gene Symbol

Tau

UniProt

P10636

NCBI Gene ID

4137

RefSeq (Protein):NP_005901.2