Tau Antibody: Biotin

• Catalog Number: orb612877
• Category: Antibodies
• Description: Mouse monoclonal antibody against Tau conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3D4
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Human Recombinant Tau441 (2N4R), P301S mutant Protein Pre-formed Fibrils
• Concentration: 1mg/mL
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• Target: Tau
• Entrez: 4137
• UniProt ID: P10636
• NCBI: NP_005901.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Tau antibody, Neurofibrillary tangle protein antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-608 was sufficient for detection of Tau 2N4R P301S Fibril in 20 ug of Mouse Brain by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Tissue: iPSC-derived neurons. Species: Human. Fixation: 4% PFA. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:100 for Overnight at 4°C. Counterstain: DAPI at 1:5000 for 5 minutes at RT in the dark. Magnification: 40X.

Western Blot analysis of Mouse Brain showing detection of Tau protein using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Lane 1: MW Marker. Lane 2: Mouse Brain (20ug). Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Dot Blot analysis using Mouse Anti-Tau Monoclonal Antibody, Clone 3D4. Tissue: Recombinant Protein. Species: Human. Primary Antibody: Mouse Anti-Tau Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:5000 for 1 hour at RT with shaking.