Tau Antibody: Biotin

• Catalog Number: orb536191
• Category: Antibodies
• Description: Rabbit polyclonal antibody against Tau conjugated to Biotin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Recombinant Human Tau 2NR4 P301S Fibril
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000)
• Conjugation: Biotin
• MW: 45.8kDa
• Target: Tau
• Entrez: 4137
• UniProt ID: P10636
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: Tau antibody, Neurofibrillary tangle protein antib
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-802 was sufficient for detection of Tau in 10 μg of human SH-SY5Y cell lysate cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-Tau Polyclonal Antibody. Tissue: Brain Cortex. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody.

Western blot analysis of SH-SY5Y showing detection of ~45.8 kDa Tau protein using Rabbit Anti-Tau Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: SH-SY5Y. Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Rabbit Anti-Tau Polyclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-rabbit IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: ~45.8 kDa. Other Band (s): 50 kDa.