TAF1 Antibody

SKU: orb345582

Description

TAF1 antibody

Images & Validation

• Tested Applications: ChIP, ELISA, IHC, WB
• Dilution range: ELISA: 1:150,000 - 1:300,000, IHC: 10µg/ml, WB: 1:100 - 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, Immunohistochemistry, ChIP, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 213 kDa in size corresponding to TAF1 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human TAF1.
• Purity: This affinity purified antibody is directed against human TAF1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography. A BLAST analysis was used to suggest cross-reactivity with TAF1 protein from human, mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.1 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Rabbit anti-TAF1 antibody, rabbit anti-Anti-Transcription Initiation Factor TFIID Subunit 1 antibody, TAF-1, TAF 1, Cell cycle gene 1 protein, TBP-associated factor 250 kDa, TAFII-250

Immunohistochemistry of rabbit anti-TAF1 antibody. Tissue: prostate. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Anti-TAF1 at 10 µg/ml for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10000 for 45 min at RT. Staining: TAF-1 as precipitated red signal with hematoxylin purple nuclear counterstain.

Transcription Initiation Factor TFIID Subunit 1 (TAF1) antibody was used to detect TAF1 in treated and untreated HeLa Cells. HeLa cells were treated with TNF alpha and Chromatin was prepared by EZ Magna Chip Kit (Millipore). CHIP was performed on fos promoters using 5 µg of TAF1 antibody from Biorbyt (P/n orb345582) and an RNA PolII antibody.

Western Blot of Rabbit anti-TAF1 antibody. Marker: Opal Pre-stained ladder. Lane 1: HEK293 lysate (p/n orb348669). Lane 2: HeLa Lysate (p/n orb348667). Lane 3: MCF-7 Lysate (p/n orb348664). Lane 4: Jurkat Lysate. Lane 5: A431 Lysate (p/n orb348665). Lane 6: A549 Lysate (p/n orb348675). Lane 7: LNCap Lysate (p/n orb348694). Lane 8: MOLT-4 Lysate (p/n orb348696). Lane 9: Ramos Lysate. Lane 10: Raji Lsyate (p/n orb348672). Lane 11: A-172 Lysate (p/n orb348708). Lane 12: NIH/3T3 Lysate (p/n orb348714). Load: 35 µg per lane. Primary antibody: TAF1 antibody at 0.2 ug/ml overnight at 4C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:30000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 250kDa for TAF1.

Western blot using Biorbyt's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1:500 (Lane 3 and 1:1000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1.

Western blot using Biorbyt's affinity purified anti-TAF1 to detect TAF1 in HeLa nuclear extract (arrowhead). The membrane was probed with the primary antibody at dilutions of 1:100 (lane 1), 1:250 (lane 2), 1:500 (Lane 3 and 1:1000 (Lane 4). The identity of the bands at ~95 kDa is unknown, but may be degraded TAF1.

Quick Database Links

UniProt

P21675

RefSeq (Protein):NP_001273003.1