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Item 1 of 4
Item 1 of 4
KAT4/TAF1 Rabbit Polyclonal Antibody
Catalog Number: orb4311
| Catalog Number | orb4311 |
|---|---|
| Category | Antibodies |
| Description | KAT4/TAF1 Rabbit Polyclonal Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF, IHC-Fr, IHC-P, WB |
| Predicted Reactivity | Bovine, Canine, Equine, Gallus, Mouse, Rat |
| Reactivity | Human |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human TAF1 |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000 |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 250-300 kDa |
| Target | TAF1 |
| UniProt ID | P21675 |
| RRID | AB_10920721 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | TBP Associated Factor 1; BA2R; CCG 1; CCG1; CCGS; Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (KAT4) Polyclonal Antibody, Unconjugated (orb4311) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KAT4) Polyclonal Antibody, Unconjugated (orb4311) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (KAT4) Polyclonal Antibody, Unconjugated (orb4311) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Sample: Lane 1: Human 293T cell lysates, Lane 2: Human HeLa cell lysates, Primary: Anti-KAT4/TAF1 (orb4311) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 200 kDa, Observed band size: 300 kDa.
