Synaptotagmin XII Polyclonal Antibody

SKU: orb1421146

Description

Rabbit polyclonal antibody to Synaptotagmin XII.

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

Synaptotagmin-12, SYT12

Sample: U-2OS (Human) Cell Lysate at 30 ug. Primary: Anti- Synaptotagmin XII at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 47 kD. Observed band size: 48 kD.

Blank control: Hela.Primary Antibody (green line): Rabbit Anti- Synaptotagmin XII antibody.Dilution: 2 µg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE. Dilution: 1 µg /test.Protocol. The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature.Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20, 000 events was performed.

Quick Database Links

UniProt

Q8IV01