Synaptotagmin XII Rabbit Polyclonal Antibody

SKU: orb158538

Description

Synaptotagmin XII Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Synaptotagmin XII (301-350/421aa)
• Target: SYT12
• Molecular Weight: 47 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

SRG1; Synaptotagmin 12; Synaptotagmin-12; Syt12; SYT12_HUMAN; SytXII.

Blank control: Hela. Primary Antibody (green line): Rabbit Anti-Synaptotagmin XII antibody (orb158538), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: U-2OS (Human) Cell Lysate at 30 ug, Primary: Anti-Synaptotagmin XII (orb158538) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 47 kD, Observed band size: 48 kD.

Quick Database Links

Gene Symbol

SYT12

UniProt

Q8IV01