Swine IgG (H&L) Antibody Peroxidase Conjugated

SKU: orb347979

Description

Swine IgG (H&L) antibody (Peroxidase)

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:50,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  Anti-Swine IgG Peroxidase conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.

Key Properties

• Antibody Type: Secondary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in rabbit.
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Swine IgG and Swine Serum.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Rabbit Anti-Swine IgG peroxidase Conjugated Antibody, Rabbit Anti-Swine IgG Antibody HRP Conjugation

ELISA Results of Rabbit Anti-Swine IgG Antibody Peroxidase Conjugate tested against purified Swine IgG HRP. Each well was coated in duplicate with 1.0 µg of Swine IgG (p/n orb2652739). The working dilution is 1:112000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer and TMB substrate (p/n orb348651).