Swine IgG (H&L) Antibody Peroxidase Conjugated

• Catalog Number: orb347966
• Category: Antibodies
• Description: Swine IgG (H&L) antibody (Peroxidase)
• Clonality: Polyclonal
• Species/Host: Goat
• Isotype: IgG
• Conjugation: HRP
• Reactivity: Porcine
• Form/Appearance: Lyophilized
• Concentration: 2.0 mg/mL
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Swine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Swine IgG and Swine Serum.
• Immunogen: Anti-Swine IgG whole molecule was produced by repeated immunization with Swine IgG whole molecule in goat.
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:100,000 - 1:200,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Application notes: Anti-Swine IgG Peroxidase conjugated has been tested by ELISA and western blot and is assayed against 1.0 µg of Swine IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
• Antibody Type: Secondary Antibody
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Alternative names: goat Anti-Swine IgG peroxidase Conjugated Antibody
• Note: For research use only

Western Blot of Peroxidase Conjugated Goat Anti-Swine IgG Secondary Antibody. Lane 1: Swine IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase goat secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Swine IgG. Other band(s): None.