Superoxide Dismutase antibody

• Catalog Number: orb344843
• Category: Antibodies
• Description: Superoxide Dismutase antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Human
• Isotype: IgG
• Immunogen: Superoxide Dismutase [Bovine erythrocytes]
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Superoxide Dismutase (SOD) is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Superoxide Dismutase [Bovine erythrocytes]. Cross reactivity against Superoxide Dismutase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P00442
• NCBI: DAA33706.1
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Superoxide Dismutase 1 Antibody, rabbi
• Note: For research use only
• Application notes: Superoxide Dismutase (SOD) Antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Superoxide Dismutase [Bovine erythrocytes] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:50,000 is suggested for this product.
• Expiration Date: 12 months from date of receipt.

References

García-Sanz, Patricia et al. N370S-GBA1 mutation causes lysosomal cholesterol accumulation in Parkinson's disease Mov Disord, 32, 1409-1422 (2017)

PubMed: 28779532