Cart summary

You have no items in your shopping cart.

SOD1 Rabbit Polyclonal Antibody

Catalog Number: orb11394

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11394
CategoryAntibodies
DescriptionSOD1 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Equine, Porcine
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human SOD1 (101-154/154aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=1ug/test
Form/AppearanceLiquid
ConjugationUnconjugated
MW17 kDa
TargetSOD1
UniProt IDP00441
RRIDAB_10752031
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesSuperoxide Dismutase 1; ALS 1; ALS; ALS1; Amyotrop
Read more...
NoteFor research use only
Expiration Date12 months from date of receipt.
SOD1 Rabbit Polyclonal Antibody

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-SOD1 antibody (orb11394), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

SOD1 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Ice-cold methanol at -20°C for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (SOD1) polyclonal Antibody, Unconjugated (orb11394) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

SOD1 Rabbit Polyclonal Antibody

Sample: Lane 1: Kidney (Mouse) Lysate at 40 ug, Lane 2: Liver (Mouse) Lysate at 40 ug, Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-SOD1 (orb11394) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 17 kD, Observed band size: 19 kD.

SOD1 Rabbit Polyclonal Antibody

Tissue/Cell: human liver cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-SOD1 Polyclonal Antibody, Unconjugated (orb11394) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

  • SOD1 Rabbit Polyclonal Antibody [orb186008]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Bovine, Equine, Porcine

    Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • SOD (Mn) Antibody: APC [orb151444]

    ICC,  IF,  IHC

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Invertebrate, Monkey, Mouse, Porcine, Rabbit, Rat, Rodent, Sheep

    Rabbit

    Polyclonal

    APC

    100 μg
  • SOD (Mn) Antibody: Biotin [orb151445]

    ELISA,  ICC,  IF,  IHC,  WB

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Invertebrate, Monkey, Mouse, Porcine, Rabbit, Rat, Rodent, Sheep

    Rabbit

    Polyclonal

    Biotin

    100 μg
  • SOD (Mn) Antibody: FITC [orb151446]

    ICC,  IF,  IHC

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Invertebrate, Monkey, Mouse, Porcine, Rabbit, Rat, Rodent, Sheep

    Rabbit

    Polyclonal

    FITC

    100 μg
  • SOD (Mn) Antibody: HRP [orb151447]

    ELISA,  IHC,  WB

    Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Invertebrate, Monkey, Mouse, Porcine, Rabbit, Rat, Rodent, Sheep

    Rabbit

    Polyclonal

    HRP

    100 μg