SUMO2/3 Antibody (C-term)

• Catalog Number: orb1937625
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This SUMO2/3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-81 amino acids from the C-terminal region of human SUMO2/3.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Hamster, Other, Porcine, Zebrafish
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: P55854
• MW: 11637 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:100, IF: 1:100, WB: 1:2000, IHC-P: 1:50~100
• Clone Number: RB00635
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Small ubiquitin-related modifier 3, SUMO-3, SMT3 h
• Note: For research use only
• NCBI: NP_008867.2

All lanes: Anti-SUMO2/3 Antibody (C-term) at 1:2000 dilution. Lane 1: SH-SY5Y whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: Jurkat whole cell lysate. Lane 4: HL-60 whole cell lysate. Lane 5: Mouse cerebellum tissue lysate. Lane 6: Mouse liver tissue lysate. Lane 7: Rat liver tissue lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 12 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with SUMO2/3 Antibody (C-term). diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Fluorescent image of SH-SY5Y cells stained with SUMO2/3 Antibody (C-term). diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.