SUMO2/3 Antibody (C-term E69)

• Catalog Number: orb30227
• Category: Antibodies
• Description: Purified rabbit polyclonal antibody.
• Target: SUMO3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Hamster, Mouse, Other, Porcine, Rat, Zebrafish
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Immunogen: Synthetic Peptide
• UniProt ID: P55854
• MW: 11637
• Tested applications: IF, IHC-P, WB
• Dilution range: WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50
• Clone Number: RB15398
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Anti-Small ubiquitin-like modifier antibody, anti-
• Research Area: Cell Biology
• Note: For research use only
• NCBI: NP_008867.2

Fluorescent confocal image of Hela cell stained with SUMO2/3 Antibody (C-term E69). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO2/3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SUMO2/3 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SUMO2/3 Antibody (C-term E69) western blot analysis in 293, Hela cell line lysates (35 ug/lane). This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).