SUMO2/3 Antibody (C-term E69)

SKU: orb30227

Description

Purified rabbit polyclonal antibody.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB: 1:1000, IF/ICC: 1:10-50, IHC-P: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Hamster, Mouse, Other, Porcine, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB15398
• Immunogen: Synthetic Peptide
• Target: SUMO3
• Molecular Weight: 11637
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Anti-Small ubiquitin-like modifier antibody, anti-Ubiquitin-like protein SMT3B antibody, anti-Sentrin 2 antibody, anti-HSMT3 antibody

Fluorescent confocal image of Hela cell stained with SUMO2/3 Antibody (C-term E69). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SUMO2/3 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SUMO2/3 immunoreactivity is localized to Nucleus significantly.

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with SUMO2/3 antibody (C-term E69), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

SUMO2/3 Antibody (C-term E69) western blot analysis in 293, Hela cell line lysates (35 ug/lane). This demonstrates the SUMO2/3 antibody detected the SUMO2/3 protein (arrow).

Quick Database Links

Gene Symbol

SUMO3

UniProt

P55854

RefSeq (Protein):NP_008867.2