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SUMO1 Antibody

Catalog Number: orb749393

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749393
CategoryAntibodies
DescriptionThis antibody is specific to SUMO1 and shows no cross-reaction with either SUMO2 or SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1 protein localizes to the nuclear membrane.
Species/HostMouse
ClonalityMonoclonal
Clone NumberSM1/495
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Rat
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human SUMO1 protein was used as the immunogen for this antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis SUMO1 antibody is available for research use only.
Entrez7341
StorageStore the SUMO1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
SUMO1 Antibody

IHC staining of FFPE human tonsil with SUMO1 antibody (clone SM1/495).

SUMO1 Antibody

Western blot testing of human HeLa cell lysate using SUMO1 antibody (clone SM1/495).

SUMO1 Antibody

Immunofluorescent testing of PFA-fixed human HepG2 cells with SUMO1 antibody (green, clone SM1/495) and Phalloidin (red).

SUMO1 Antibody

Flow cytometry testing of PFA-fixed human HepG2 cells with SUMO1 antibody (clone SM1/495); Red = isotype control, Blue = SUMO1 antibody.

SUMO1 Antibody

SDS-PAGE analysis of purified, BSA-free SUMO1 antibody (clone SM1/495) as confirmation of integrity and purity.

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