SUMO1 Antibody

SKU: orb2641670

Description

The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability.

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Rat
• Application Notes:
  Titering of the SUMO1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: S1MT-2
• Immunogen: Recombinant human protein was used as the immunogen for this SUMO1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot testing of SUMO1 antibody and human HeLa lysate (clone S1MT-2).

IHC staining of human tonsil with SUMO1 antibody (clone S1MT-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC staining of rat pancreas with SUMO1 antibody (clone S1MT-2). Required HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

Immunofluorescent testing of PFA-fixed human HepG2 cells with SUMO1 antibody (green, clone S1MT-2) and Phalloidin (red).