UBASH3B Antibody

SKU: orb345502

Description

Sts-1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IHC: 4µg/ml, WB: 1:500 - 1:3,000
• Reactivity: Human, Mouse
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, IHC, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-terminus of mouse Sts-1.
• Target: UBASH3B
• Purity: This affinity purified antibody is directed against mouse Sts-1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from mouse, human, rat and dog based on 100% homology for the immunogen sequence. Cross reactivity with Sts-1 homologues from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.95 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Sts-1 antibody, Sts1, Sts 1, Ubiquitin-associated and SH3 domain-containing protein B, Cbl interacting protein p70 antibody, Suppressor of T-cell receptor signaling 1, T-cell ubiquitin ligand 2, TULA-2, Tyrosine-protein phosphatase STS1/TULA2, Ubash3B

Immunohistochemistry with anti-Sts-1 antibody showing Sts-1 staining of histiocytic elements in cytoplasm of human tonsil at 20x and 40x (B & C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH6.2 and then incubated with rabbit anti-Sts-1 antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using MACH 1 universal HRP polymer detection system and visualized with 3'3-diamino-benzidine substrate (DAB).

Western blot using Biorbyt's Affinity Purified anti-Sts-1 antibody shows detection of a band ~70 kDa corresponding to mouse Sts-1. Approximately 1.0 µg of recombinant (truncated) Sts-1-GST was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 0.3 ug/ml overnight at 4°C followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX.

Quick Database Links

Gene Symbol

UBASH3B

UniProt

Q8TF42

RefSeq:24497612