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Catalog Number | orb1256563 |
---|---|
Category | Antibodies |
Description | UBASH3B Antibody |
Target | UBASH3B |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human UBASH3B (NP_116262.2). |
UniProt ID | Q8TF42 |
MW | Observed: 72kDa |
Tested applications | IF, IHC, WB |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | STS-1, STS1, TULA-2, TULA2, p7ubiquitin-associated Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using UBASH3B antibody (orb1256563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat spleen using UBASH3B antibody (orb1256563) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using UBASH3B antibody (orb1256563). Blue: DAPI for nuclear staining.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Bovine, Canine, Equine, Guinea pig, Human, Mouse, Rabbit, Zebrafish | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |