STIM1 Antibody

• Catalog Number: orb1240038
• Category: Antibodies
• Description: STIM1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: STIM1 antibody was raised against a 24 amino acid synthetic peptide from near the carboxy terminus of human STIM1.The immunogen is located within the last 50 amino acids of STIM1.
• Concentration: 1 mg/mL
• Dilution range: STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 59, 75, 87 kDa Observed: 88 kDa
• Target: STIM1
• UniProt ID: Q13586
• NCBI: NP_003147
• Storage: STIM1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: STIM1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: STIM1 Antibody: GOK, TAM, IMD10, D11S4896E, GOK, S
• Note: For research use only
• Application notes: STIM1 antibody can be used for detection of STIM1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of STIM1 in mouse thymus tissue lysate with STIM1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemistry of STIM1 in human spleen tissue with STIM1 antibody at 2.5 µg/mL.

Immunohistochemistry of STIM1 in mouse spleen tissue with STIM1 antibody at 5 µg/ml.

Immunofluorescence of STIM1 in mouse spleen tissue with STIM1 antibody at 20 µg/mL. Green: STIM1 Antibody (orb1240038) Blue: DAPI staining.

Immunofluorescence of STIM1 in Hek293 cells with STIM1 antibody at 20 µg/mL. Red: STIM1 Antibody (orb1240038) Blue: DAPI staining.