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Item 1 of 2
Anti-STIM1 Purified
Catalog Number: orb43733
| Catalog Number | orb43733 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to STIM1. |
| Clonality | Monoclonal |
| Clone Number | CDN3H4 |
| Tested applications | ICC, IHC-P, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Mouse IgG1 |
| Immunogen | Synthetized peptide (C-terminal cytoplasmic part of STIM1). |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | STIM1 |
| Entrez | 6786 |
| UniProt ID | G0XQ39 |
| RRID | AB_10991250 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Alternative names | STIM1 antibody, anti-GOK Read more... |
| Note | For research use only |
| Application notes | Immunocytochemistry: Methanol-aceton fixation; positive control: HeLa human cervix carcinoma cell line.Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml.Western blotting: Recommended dilution: 1 μg/ml; positive control: RBL rat basophilic leukemia cell line; both reducing and non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Western blotting analysis (non-reducing conditions) of whole cell lysate of RBL rat basophilic leukemia cell line. Lane 1: immunostaining with anti-STIM1 (CDN3H4); Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06; cat. no. orb43531).
Immunocytochemistry staining of HeLa human cervix carcinoma cell line using anti-STIM1 (CDN3H4; methanol-aceton fixation; detection by Goat anti-mouse IgG1 Alexa Fluor® 598; red). Nuclei were stained with DAPI (blue).
