Stat5a Antibody

SKU: orb344455

Description

STAT5 (phospho-Y694) antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:20,000, IHC: 20-40 µg/ml, IF: 1:50 - 1:1,000, WB: 1:500 - 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  This monoclonal antibody has been tested by ELISA, immunofluorescence, immunohistochemistry, and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a phospho Y694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5F6.F1
• Immunogen: Phospho STAT5 pY694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Target: Stat5a
• Purity: Anti-STAT5 phospho Y694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store Anti-STAT5 phospho Y694 Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Phospho STAT5 pY694 Antibody is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody

Quick Database Links

Gene Symbol

Stat5a

UniProt

P42230

RefSeq:6755672