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STAT5 Rabbit Polyclonal Antibody

Catalog Number: orb11432

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11432
CategoryAntibodies
DescriptionSTAT5 Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityBovine, Canine, Equine, Porcine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human STAT5 (61-160/794aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500, Flow-Cyt=2ug/Test, ELISA=1:5000-10000
Form/AppearanceLiquid
ConjugationUnconjugated
MW90 kDa
TargetSTAT5A
UniProt IDP42229
RRIDAB_10753285
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namessignal transducers and activators of transduction
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NoteFor research use only
Expiration Date12 months from date of receipt.
STAT5 Rabbit Polyclonal Antibody

Blank control: Jurkat. Primary Antibody (green line): Rabbit Anti-STAT5 antibody (orb11432), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

STAT5 Rabbit Polyclonal Antibody

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (STAT5) polyclonal Antibody, Unconjugated (orb11432) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

STAT5 Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human endometrial carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (STAT5) Polyclonal Antibody, Unconjugated (orb11432) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

STAT5 Rabbit Polyclonal Antibody

Tissue/Cell: Mouse lymph tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-STAT5 Polyclonal Antibody, Unconjugated (orb11432) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    IHC,  WB

    Bovine, Canine, Equine, Goat, Guinea pig, Mouse, Porcine, Rabbit, Rat, Sheep, Zebrafish

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
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    ELISA,  IF,  IHC-P,  WB

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    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl
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    IF,  IH,  IP,  WB

    Human, Primate

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl
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    IH,  WB

    Human, Mouse, Porcine, Rat, Sheep

    Rabbit

    Polyclonal

    Unconjugated

    30 μl, 100 μl, 200 μl