Stat5 phospho Y694 Antibody

SKU: orb344440

Description

STAT5 (phospho-Y694) antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC, Multiplex Assay, WB
• Dilution Range: ELISA: 1:20,000, IHC: 20-40 µg/ml, IF: 1:50 - 1:1,000, WB: 1:500 - 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 5F6.F1
• Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Purity: Phospho STAT5 pY694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-STAT5 pY694 Antibody, MGF antibody, Signal Transducer and Activator of Transcription 5A antibody, STAT 5 antibody

Immunofluorescent STED Microscopy of Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody in 3T3 cells. Anti-Stat5 pY694 at 1:500 detecting Stat5 in 3T3 cells. Staining: Stat5 pY694 protein [Red]. Tubulin [Green].

Immunohistochemistry of Monoclonal Anti-Stat5 pY694 Antibody. Tissue: human breast tissue (40X). Fixation: formalin fixed paraffin embedded (FFPE). Antigen retrieval: steam sections in 0.1 M sodium citrate buffer, pH6, for 20 min. Rinse with 1XTBST. Primary antibody: Anti-Stat5pY694 at 20 µg/ml. Localization: breast epithelium with moderate nuclear staining. Staining: Stat5 pY694 as precipitated red signal, with a hematoxylin purple nuclear counterstain.

Western blot using Biorbyt's Protein A purified Mouse Monoclonal anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1000 dilution, overnight at 4°C. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20000 dilution for 30 min at room temperature.

Quick Database Links

UniProt

P42230

RefSeq:6755672