STAT5 (phospho-Y694) antibody

• Catalog Number: orb344440
• Category: Antibodies
• Description: STAT5 (phospho-Y694) antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5F6.F1
• Tested applications: ELISA, IF, IHC, Multiplex Assay, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Anti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:20,000, IHC: 20-40 µg/ml, IF: 1:50 - 1:1,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Phospho STAT5 pY694 Antibody was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for mouse Stat5a protein phosphorylated at Y694. A BLAST analysis was used to suggest cross-reactivity with Stat5a from human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Stat5a from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: P42230
• NCBI: 6755672
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: mouse anti-STAT5 pY694 Antibody, MGF antibody, Sig
• Note: For research use only
• Application notes: Phospho STAT5 pY694 monoclonal antibody has been tested by ELISA, western blot, immunohistochemistry, and immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to phosphorylated Stat5a protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with mouse Stat5a pY694 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of 3T3 cells using STAT5 (phospho-Y694) antibody

Immunohistochemical staining of human breast tissue using STAT5 (phospho-Y694) antibody

Western blot analysis of NK92 cells using STAT5 (phospho-Y694) antibody