STAT3 Rabbit mAb [KD Validated]

• Catalog Number: orb1951799
• Category: Antibodies
• Description: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human STAT3
• UniProt ID: P40763
• Tested applications: ICC, IHC, WB
• Dilution range: WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
• Antibody Type: Recombinant Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APRF;Acute-phase response factor;Signal transducer
• Note: For research use only

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells treated with IFNalpha, using STAT3 Antibody.

Immunohistochemical analysis of paraffin-embedded rat brain, using STAT3 Antibody.

Western blot analysis of STAT3 expression in (1) A431 cell lysate; (2) Human liver lysate; (3) Mouse heart lysate; (4) Rat brain lysate.