STAT3 (Phospho-Tyr705) Rabbit mAb [KD Validated]

• Catalog Number: orb1727718
• Category: Antibodies
• Description: The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human STAT3
• Antibody Type: Recombinant Antibody
• Dilution range: WB:1:5000~1:10000IHC:1:50~1:100ICC/IF:1:50~1:000IP:1:30
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• MW: 88kDa
• UniProt ID: P40763
• Source: Recombinant Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: APRF; Stat3; HIES; Acute-phase response factor;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

Immunofluorescent analysis of HeLa cells treated with IFN-alpha, using Phospho-STAT3 (Y705) Antibody.

Immunohistochemical analysis of paraffin-embedded human pancreas, using Phospho-STAT3 (Y705) Antibody.

Western blot analysis of Phospho-STAT3 (Tyr705) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with IFN-a.