SQSTM1 Antibody

SKU: orb1274537

Description

SQSTM1 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, IP, KO/KD Validated, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:100IP: 1:20 - 1:50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human SQSTM1 / p62 (NP_001135770.1).
• Target: SQSTM1
• Molecular Weight: Observed: 62kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SQSTM1 Antibody: p60, p62, A170, OSIL, PDB3, ZIP3, p62B, ORCA, Sequestosome-1, EBI3-associated protein of 60 kDa, EBIAP

Western blot analysis of extracts of various cell lines, using SQSTM1 antibody (orb1274537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Western blot analysis of extracts from normal (control) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody (orb1274537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human esophagus using SQSTM1/p62 antibody (orb1274537) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using SQSTM1/p62 antibody (orb1274537) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100 ug extracts of HepG2 cells using 3 ug SQSTM1/p62 antibody (orb1274537). Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody (orb1274537) at a dilution of 1:1000.

Quick Database Links

Gene Symbol

SQSTM1

UniProt

Q13501