Sprouty Antibody

• Catalog Number: orb1246912
• Category: Antibodies
• Description: Sprouty Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: The immunogen for this antibody is: CPSRGQGKPS
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SPRY1
• UniProt ID: O43609
• NCBI: NP_955359.1, NP_005832.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SPRY1, hSPRY1, sprouty homolog 1, antagonist of FG
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Kidney lysates (calculated MW of 35.1kDa according to NP_005832.1 ). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS cells (PMID: 20068162). Recommended concentration: 1-3ug/ml.Immunohistochemistry:This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246912 (0.3 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246912 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic and Golgi apparatus staining.

orb1246912 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing cytoplasmic and Golgi apparatus staining.

orb1246912 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (0.4 ug/ml). IgG control: Unimmunized goat IgG (black line).