SPON2 Antibody

• Catalog Number: orb1246824
• Category: Antibodies
• Description: SPON2 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Canine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RTRYVRVQPANNGSP
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line A549 (calculated MW of 35.8kDa according to NP_036577.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 35 kDa
• Target: SPON2
• UniProt ID: Q9BUD6
• NCBI: NP_036577.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SPON2, spondin 2, extracellular matrix protein, DI
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 35kDa band observed in lysates of cell line A549 (calculated MW of 35.8kDa according to NP_036577.1). Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246824 (1 ug/ml) staining of A549 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246824 (5 ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.