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| Catalog Number | orb12991 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to SPON2. |
| Target | SPON2 (301-315) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Canine |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | RTRYVRVQPANNGSP |
| MW | 35.8 |
| Tested applications | ELISA, IHC, WB |
| Dilution range | ELISA: 1:16000, WB: 0.5-2 μg/ml, IHC-P: 5 μg/ml |
| Application notes | WB: Approx 35-36kDa band observed in Human, Mouse and Rat Kidney lysates, and in preliminary testing of A549 cell lysates (calculated MW of 35.8kDa according to Human NP_036577.2,and 36.0kDa according to Mouse NP_598664.3 and Rat NP_612542.1). Recommended con. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5µg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti SPON2 antibody |
| Note | For research use only |
| Entrez | 10417 |

Western blot analysis of Human (Lane 1), Mouse (Lane 2) and Rat (Lane 3) Kidney lysate using SPON2 antibody.

Immunohistochemical staining of Human Tonsil of SPON2 antibody.
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