Spinesin Antibody

• Catalog Number: orb1246913
• Category: Antibodies
• Description: Spinesin Antibody
• Target: TMPRSS5
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: C-LDWIHDTAQDSLL
• UniProt ID: Q9H3S3
• Tested applications: ELISA, IHC, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex). These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5ug/ml.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TMPRSS5, SPINESIN, transmembrane protease, serine
• Research Area: Cancer Biology
• Note: For research use only
• NCBI: NP_110397.2

orb1246913 staining (0.5 ug/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1246913 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246913 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.