TMPRSS5 Antibody

SKU: orb1246913

Description

TMPRSS5 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex). These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5µg/mL.Immunohistochemistry:Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-LDWIHDTAQDSLL
• Target: TMPRSS5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TMPRSS5, SPINESIN, transmembrane protease, serine 5 (spinesin), MGC141886, MGC148044, SPINESIN

orb1246913 staining (0.5 ug/ml) of Human Brain (Cerebral Cortex) lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

orb1246913 (5 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246913 (5 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

TMPRSS5

UniProt

Q9H3S3

RefSeq (Protein):NP_110397.2