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Spinesin/TMPRSS5 Antibody

SKU: orb18346

Description

Goat polyclonal antibody to TMPRSS5

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 0.5-1.5 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 50kDa and 29kDa in Human Brain (Cerebral Cortex) lysates and in Mouse and Rat Brain lysates after 0.5 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Yamaguchi et al, J Biol Chem. 2002 Mar 1;277(9):6806-12; PMID: 11741986). This protein has a calculated MW of 49.6kDa according to NP_110397.1. Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

ClonalityPolyclonal
TargetSpinesin / TMPRSS5
Protein SequenceLDWIHDTAQDSLL
Molecular Weight49.6
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti TMPRSS5 antibody, anti SPINESIN antibody, anti transmembrane protease, serine 5 (spinesin) antibody, anti MGC141886 antibody, anti MGC148044 antibody, anti SPINESIN antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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