SPHK1 Antibody (N-term P74)

• Catalog Number: orb1929452
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB15394
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, IHC-P: 1:100, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 42518 Da
• Target: This SPHK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-89 amino acids from the N-terminal region of human SPHK1.
• UniProt ID: Q9NYA1
• NCBI: NP_001136074.1, NP_892010.2, NP_068807.2, NP_001136073.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sphingosine kinase 1, SK 1, SPK 1, SPHK1, SPHK, SP
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. kidney section using SPHK1 Antibody (N-term P74). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded M. kidney section using SPHK1 Antibody (N-term P74). Diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of lysates from HepG2 cell line and human heart, skeletal muscle, kidney tissue lysate (from left to right), using SPHK1 Antibody (N-term P74). Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.