SPECC1L Antibody

SKU: orb1269778

Description

SPECC1L Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Zebrafish
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1.
• Target: SPECC1L
• Molecular Weight: 125 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

Cytospin-A, Renal carcinoma antigen NY-REN-22, Sperm antigen with calponin homology and coiled-coil domains 1-like, SPECC1-like protein, SPECC1L, CYTSA, KIAA0376

Western blot analysis in HL-60 cell line lysates (35 ug/lane).

Western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane).

SPECC1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of SPECC1 Antibody with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Quick Database Links

Gene Symbol

SPECC1L

UniProt

Q69YQ0

RefSeq:Q69YQ0