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Catalog Number | orb1937711 |
---|---|
Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Target | This SPECC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 705-733 amino acids from the Central region of human SPECC1. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit IgG |
Conjugation | Unconjugated |
Reactivity | Human, Mouse |
Predicted Reactivity | Zebrafish |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
UniProt ID | Q69YQ0 |
MW | 124544 Da |
Tested applications | FC, IF, IHC-P, WB |
Dilution range | IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50 |
Antibody Type | Primary Antibody |
Clone Number | RB31720 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Cytospin-A, Renal carcinoma antigen NY-REN-22, Spe Read more... |
Note | For research use only |
NCBI | NP_056145.4, NP_001241661.2 |
Confocal immunofluorescent analysis of SPECC1 Antibody (Center) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
SPECC1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
SPECC1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of SPECC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
SPECC1 Antibody (Center) western blot analysis in HL-60 cell line lysates (35 ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).
SPECC1 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the SPECC1 antibody detected the SPECC1 protein (arrow).