SPANXC Antibody

SKU: orb345533

Description

SPANX antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000
• Reactivity: Human
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~97kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 25-50 of human SPANX protein.
• Target: SPANXC
• Purity: This affinity-purified antibody is directed against human SPANX proteins. The immunogen sequence is present within SPANX-A, SPANX-B, SPANX-C, SPANX-D and SPANX-N, but not present within SPANX-E. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with SPANX proteins from gorilla and chimpanzee. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-SPANX antibody, SPANXC, SPANX-C, Sperm protein associated with the nucleus on the X chromosome C, Cancer-testis-associated protein CTp11 antibody, Cancer/testis antigen 11.3, CTP11 antibody, Nuclear associated protein SPAN Xc antibody, SPANX family member C antibody

Western blot using Biorbyt's affinity purified anti-SPANX (pan) antibody shows detection of a band at ~17kDa corresponding to SPANX-C present in a nuclear extract from VWM105 cells in Lane 1. VWM105 cells are derived from a human melanoma and are positive for SPANX proteins. Lane 2 shows reactivity with a purified recombinant SPANX-C fusion protein ~97kDa. The right panel shows similar reactivity with purified recombinant SPANX-B, SPANX-C and SPANX-N proteins. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1000. IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX was used (left).

Quick Database Links

Gene Symbol

SPANXC

UniProt

Q9NY87

RefSeq:57471638