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Catalog Number | orb344633 |
---|---|
Category | Antibodies |
Description | SPANX-C antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein. |
Concentration | 5.0 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:20,000, IHC: 1.25-2.5 µg/ml, WB: 1:1,000 - 1:5,000 |
Form/Appearance | Lyophilized |
Purity | This Protein A purified antibody is directed against human SPANX-C protein. The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above. A protein-protein BLAST analysis was used to suggest cross reactivity with most SPANX proteins from human, gorilla and chimpanzee. Reactivity against homologues from is not known. |
Conjugation | Unconjugated |
UniProt ID | Q9NY87 |
NCBI | 6977870 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-SPANX-C antibody, Cancer-testis-associ Read more... |
Note | For research use only |
Application notes | This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue. Most human cell lines are SPANX negative. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical staining of multi-human, multi-brain and multi-cancer slides using SPANX-C antibody
Western blot analysis of human melanoma tissue using SPANX-C antibody
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