SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) Antibody

• Catalog Number: orb1410067
• Category: Antibodies
• Description: Mouse monoclonal antibody to SOX4 (Master Regulator of Epithelial-Mesenchymal Transition)
• Target: SOX4
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Purification: Protein A
• Immunogen: Recombinant fragment (around aa45-130) of human SOX4 protein
• UniProt ID: Q06945
• MW: 47kDa
• Tested applications: FC, IF, IHC, WB
• Clone Number: PCRP-SOX4-1D6
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SOX; SRY (sex determining region Y) box 4; SRY box
• Research Area: Cardiovascular Research, Cell Biology, Signal Tran
• Note: For research use only

Analysis of Protein Array containing more than 19000 full-length human proteins using SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Flow Cytometric Analysis of PFA-fixed HeLa cells. SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Formalin-fixed, paraffin-embedded human breast carcinoma stained with SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Formalin-fixed, paraffin-embedded human colon carcinoma stained with SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). HIER: Tris/EDTA, pH9.0, 45 min. 2°: HRP-polymer, 30 min. DAB, 5 min.

Immunofluorescence Analysis of PFA-fixed HeLa cells stained using SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).

SDS-PAGE Analysis Purified SOX4 Mouse Monoclonal Antibody (PCRP-SOX4-1D6). Confirmation of Purity and Integrity of Antibody.