SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) antibody

• Catalog Number: orb1410067
• Category: Antibodies
• Description: Mouse monoclonal antibody to SOX4 (Master Regulator of Epithelial-Mesenchymal Transition)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-SOX4-1D6
• Tested applications: FC, IF, IHC, IP, WB
• Reactivity: Gallus, Human
• Isotype: Mouse / IgG2b, kappa
• Immunogen: Recombinant fragment (around aa45-130) of human SOX4 protein
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunoprecipitation (1-2ug per 100-500ug of total protein (1ml of cell lysate)); ,Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); ,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Conjugation: Unconjugated
• MW: 47kDa
• Target: SOX4
• Entrez: 6659
• UniProt ID: Q06945
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: SOX; SRY (sex determining region Y) box 4; SRY box
• Note: For research use only
• Expiration Date: 12 months from date of receipt.