SORBS1 Antibody

SKU: orb1246920

Description

SORBS1 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-SERNDWEPPDKKVD
• Target: SORBS1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SORBS1, sorbin and SH3 domain containing 1, CAP, DKFZp451C066, DKFZp586P1422, FLAF2, FLJ12406, KIAA1296, R85FL, SH3D5, SH3P12, SORB1 , Fas-ligand associated factor 2, SH3-domain protein 5, SH3-domain protein 5 (ponsin), c-Cbl associated protein, ponsin

orb1246920 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246920 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

SORBS1

UniProt

Q9BX66

RefSeq (Protein):NP_006425.2, NP_001030127.1, NP_001030128.1, NP_056200.1, NP_079267.1, NP_001030129.1, NP_001030126.1