SORBS1 Antibody

• Catalog Number: orb1246920
• Category: Antibodies
• Description: SORBS1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Predicted Reactivity: Mouse
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-SERNDWEPPDKKVD
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SORBS1
• UniProt ID: Q9BX66
• NCBI: NP_006425.2, NP_001030129.1, NP_001030127.1, NP_079267.1, NP_001030128.1, NP_001030126.1, NP_056200.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SORBS1, sorbin and SH3 domain containing 1, CAP, D
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Pancreas and Liver. Recommended concentration: 3.75ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246920 (3.75 ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246920 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.