SORBS1/ponsin (internal region) Antibody

SKU: orb19512

Description

Goat polyclonal antibody to SORBS1

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 65kDa band in Human Adipose lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 76.6kDa according to NP_006425.2 and higher sizes for other isoforms. The 65kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SORBS1/ponsin (internal region)
• Molecular Weight: 76.6; 90.2; 142; 127; 101; 87.2; 83.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

SORBS1; sorbin and SH3 domain containing 1; CAP; DKFZp451C066; DKFZp586P1422; FLAF2; FLJ12406; KIAA1296; R85FL; SH3D5; SH3P12; SORB1 ; Fas-ligand associated factor 2; SH3-domain protein 5; SH3-domain protein 5 (ponsin); c-Cbl associated protein; ponsin

Immunohistochemical staining of Human Pancreas using SORBS1 antibody

Immunohistochemical staining of Human Liver using SORBS1 antibody

Quick Database Links

Gene Symbol

SORBS1/ponsin (internal region)

NCBI Gene ID

10580