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SOD (Cu/Zn) Antibody: HRP

Catalog Number: orb151413

DispatchUsually dispatched within 2-3 weeks
$ 600.00
Catalog Numberorb151413
CategoryAntibodies
DescriptionRabbit polyclonal to SOD (HRP). Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space. SOD3 can also be distinguished by its heparin-binding capacity..
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityBovine, Fungi, Human, Mouse, Rat
ImmunogenSynthetic Rat Cu/Zn SOD Peptide
Concentration1 mg/ml
Dilution rangeWB (1:2000), ICC/IF (1:120)
ConjugationHRP
MW19kDa
TargetSOD1
Entrez24786
UniProt IDP07632
NCBINP_058746.1
StorageConjugated antibodies should be stored according to the product label
Buffer/Preservatives73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
Alternative namesSuperoxide dismutase1 antibody, ALS1 antibody, IPO
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NoteFor research use only
Application notes0.5 µg/ml of SPC-115 was sufficient for detection of Cu/Zn SOD in 20 µg of rat brain tissue extract by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.
Expiration Date12 months from date of receipt.
SOD (Cu/Zn) Antibody: HRP

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody at 1:120 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

SOD (Cu/Zn) Antibody: HRP

Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

SOD (Cu/Zn) Antibody: HRP

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD (Cu/Zn) Polyclonal Antibody at 1:120 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rabbit (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD (Cu/Zn) Antibody. (C) Composite.

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