SOD1 (Cu/Zn) Antibody (Biotin)

SKU: orb151428

Description

Rabbit polyclonal to SOD (Biotin). Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extracellular space(9). SOD3 can also be distinguished by its heparin-binding capacity..

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution range: WB (1:5000), IHC (1:100)
• Reactivity: Bovine, Canine, Fish, Frog, Hamster, Human, Invertebrate, Monkey, Mouse, Mussel, Other, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  0.2 µg/ml of SPC-116 was sufficient for detection of Cu/Zn SOD in 20 µg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic Human Cu/Zn SOD Peptide
• Target: SOD1 (Cu/Zn)
• Molecular Weight: 19kDa
• Purification: Protein A Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SOD1, ALS1, SODC_HUMAN, Superoxide dismutase1, IPOA, SODC

Immunohistochemistry analysis using Rabbit Anti-SOD1 Polyclonal Antibody. Tissue: Placenta. Species: Human. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody at 1:100.

Western blot analysis of Human Cell line lysates showing detection of SOD1 protein using Rabbit Anti-SOD1 Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA. Primary Antibody: Rabbit Anti-SOD1 Polyclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Quick Database Links

Gene Symbol

SOD1 (Cu/Zn)

UniProt

P00441

NCBI Gene ID

6647

RefSeq (Protein):NP_000445.1