SOD1 (UbetaB) Antibody (Biotin)

SKU: orb152254

Description

Rabbit polyclonal to SOD1 (Biotin). Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body. It works by catalyzing the dismutation of the superoxide radical O2 to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (1,4). In general, SODs play a major role in antioxidant defense mechanisms. There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge. Misfolding of SOD1 has been implicated in Amyotrophic lateral sclerosis (ALS). Therefore conformation specific antibodies such as Anti-SOD1 (U beta B), which targets an unfolded region of the beta barrel of SOD1, are useful for determining the conformation of SOD1 in affected tissues. This antibody can be used in conjunction with Anti-SOD1 (EDI) which detects an exposed dimer interface (EDI) of SOD1..

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Dilution range: WB (1:1000), ICC/IF (1:100)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1µg/ml of SPC-205 was sufficient for detection of the unfolded beta barrel of SOD1 by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Within 50 AA of N-terminal region of SOD1, unfolded beta barrel region
• Target: SOD1 (UbetaB)
• Purification: Peptide Affinity purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

SOD1 (UbetaB), SOD1, Superoxide dismutase [Cu-Zn], Cu/Zn superoxide dismutase, SODC_HUMAN, ALS1, USOD, SOD1 unfolded beta barrel region, Misfolded SOD1, Conformationally altered SOD1, Apo-SOD1, Superoxide dismutase 1, SOD

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody at 1:110 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cytoplasm. Nucleus. Mitochondrion. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-SOD1 (UBB) Antibody. (C) Composite.

Western blot analysis of Human, Rat, Mouse Hela cells, Brain, Lung showing detection of ~18 kDa SOD1 (UBB) protein using Rabbit Anti-SOD1 (UBB) Polyclonal Antibody. Lane 1: Molecular Weight Ladder. Lane 2: Human HeLa Cell Lysates. Lane 3: Rat Brain. Lane 4: Mouse Lung. Load: 20 μg. Primary Antibody: Rabbit Anti-SOD1 (UBB) Polyclonal Antibody at 1:1000. Predicted/Observed Size: ~18 kDa.

Quick Database Links

Gene Symbol

SOD1 (UbetaB)

UniProt

P00441

NCBI Gene ID

6647

RefSeq:CAG46542