SNAI1 Antibody

• Catalog Number: orb1246937
• Category: Antibodies
• Description: SNAI1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Mouse
• Reactivity: Human, Rat
• Immunogen: The immunogen for this antibody is: RKPSDPNRKPNY-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in lysates of cell line A431 and in Rat Kidney lysates (calculated MW of 29.1kDa according to Human NP_005976.2 and Rat NP_446257.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Rat: Cui F et al (2015). PMID: 26417374.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat: Cui F et al (2015). PMID: 26417374.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SNAI1
• UniProt ID: O95863
• NCBI: NP_005976.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SNAI1, snail homolog 1 (Drosophila), SLUGH2, SNA,
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 28kDa band observed in lysates of cell line A431 and in Rat Kidney lysates (calculated MW of 29.1kDa according to Human NP_005976.2 and Rat NP_446257.1). Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Rat: Cui F et al (2015). PMID: 26417374.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml. This product has been successfully used in IHC on Rat: Cui F et al (2015). PMID: 26417374.
• Expiration Date: 12 months from date of receipt.

orb1246937 (0.1 ug/ml) staining of A431 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246937 (0.1 ug/ml) staining of Rat Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1246937 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.