SNAI1 Antibody (N-term D24)

SKU: orb33657

Description

Rabbit polyclonal antibody to SNAIL belongs to the snail C2H2-type zinc-finger protein family. Snail has its highest expression in kidney, regulating epithelial-to-mesenchymal transitions and cell migration. Snail family members influence cell behavior during development and diseases such as metastatic cancer, where they act primarily as survival factors and inducers of cell movement.

Research Area

Cancer Biology, Cardiovascular Research, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:1000, IHC-P - 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD23622
• Immunogen: Synthetic Peptide
• Target: SNAI1
• Molecular Weight: 29083
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-"Zinc finger protein SNAI1 antibody, anti-Snail protein homolog antibody, anti-Sna protein" antibody, anti-SNAH antibody

Western blot analysis of SNAI1 (arrow) using rabbit polyclonal hSNAI1-D24. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SNAI1 gene (Lane 2).

HepG2 cells were incubated with HGF for the time periods indicated. LiCl and MG132 were added 8 h before lysis of the cells. Snai1 protein levels and beta actin as loading control were analyzed by WB.

Formalin-fixed and paraffin-embedded human Spleen tissue reacted with SNAI1 antibody (N-term D24), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

SNAI1

UniProt

O95863

RefSeq (Protein):NP_005976.2

Wen, Yu-Ching et al. Snail as a potential marker for predicting the recurrence of prostate cancer in patients at stage T2 after radical prostatectomy Clin Chim Acta, 431, 169-173 (2014)

PubMed: 24513541

Chiu, Jen-Hwey et al. Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers J Transl Med, 15, 97 (2017)

PubMed: 28472954