Smooth Muscle Actin Antibody [SPM332]

• Catalog Number: orb1251889
• Category: Antibodies
• Description: Smooth Muscle Actin Antibody [SPM332]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM332
• Tested applications: FC, IF, IHC, WB
• Reactivity: Canine, Feline, Gallus, Guinea pig, Human, Monkey, Mouse, Primate, Rabbit, Rat
• Isotype: IgG2a, kappa
• Immunogen: A 10 amino acid peptide from the alpha smooth muscle isoform of actin was used as the immunogen for this Smooth Muscle Actin antibody.
• Antibody Type: Primary Antibody
• Concentration: 0.2 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ACTA2
• UniProt ID: P62736
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: ACTA2, ACTSA, actin, alpha 2, smooth muscle, aorta
• Note: For research use only
• Application notes: Western blot: 1-2 ug/mlFlow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 0.25-0.5 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The optimal dilution of the Smooth Muscle Actin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded Leiomyosarcoma stained with Smooth Muscle Actin antibody (SPM332).